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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which ends up in chromosome instability, and so may add to tumorigenesis. This protein was uncovered to associate with, and be associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a critical position in marketing cell-cycle progression within the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are already described. Two added splice variants have been documented but comprehensive nucleotide sequence facts just isn't yet readily available.
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Blocking peptides are peptides that bind precisely on the focus on antibody and block antibody binding. These peptide usually contains the epitope regarded through the antibody.
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This guide outlines further possible optimization of cellular and immunoassay parameters to ensure the best possible final results are...
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This guidebook outlines further more feasible optimization of cellular and immunoassay parameters to be sure the best possible final results are...
Antibodies certain to the blocking peptide not bind for the epitope within the goal protein. By comparing the staining in the blocked antibody versus the antibody on your own, one can see which staining is distinct.
Goal Details Cyclin E belongs towards the extremely conserved cyclin family members, whose users are characterised by a spectacular periodicity in protein abundance from the mobile cycle. Cyclins operate as regulators of CDK kinases. Diverse cyclins show distinct expression and degradation designs which lead on the temporal coordination of each and every mitotic function. Cyclin E varieties a posh with and features like a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S transition. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells progress via S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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